Using machine-learning algorithms and novel segmentation techniques, QuantiMus is able to identify muscle fibers and determine their cross-sectional areas and minimum feret diameters.
QuantiMus allows the user to identify the mean fluorescence intensity in each individual fiber of a muscle section. This feature can be used in conjuction with any fluorescent stain that the user chooses.
After identifying muscle fibers in a cross-section, QuantiMus is able to determine which muscle fibers are centrally nucleated.